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add TMB The chromogenic substrate is incubated at room temperature in the dark from light to develop color
spleen and skeletal muscle
molecular biology
KIM3 Accession Q8TDQ0 Amino Acid Sequence Ser22-Arg200
Mouse NPHN ELISA Kit Recombinant Protein add TMB The chromogenic substrateProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Urine Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH=7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh