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Adding substrate: Add 90 μL of substrate (TMB) to each well
103:1799– 805
avoiding contact with the sides
5 kDa Observed MW: 50-60 kDa Purity ＞95% by SDS-PAGE Endotoxin <0
Human MGL ELISA Kit Luciferase Test Kit Adding substrate: Add 90 μLProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
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